Cadenza
Cadenza CW-C18 - Wide pore에 의한 고속 분리 ODS 컬럼
Features
● 와이드 포어 (30 nm) 실리카 ODS 컬럼 ● 독자적인 고분자 엔드캡핑 기술 적용 ● 항생제, 염기성 화합물, 인지질, 세라마이드 분석에 최적화 ● 분자량이 큰 화합물의 효율적 분리 ● 고처리량 (High-throughput) 분석 지원 ● 다양한 컬럼 사이즈 제공 : 내경 (ID) 0.075 ~ 28 mm / 길이 10 ~ 500 mm
SPECIFICATIONS
Main Specifications
| Particle size | 3 μm (Cadenza CW-C18) 5 μm (Cadenza 5CW-C18) |
|---|---|
| Base materials | 완전다공성 고순도 실리카겔 (Fully porous, high-purity silica gel) |
| Pore size | 30 nm |
| Ligand | 옥타데실기 (Octadecyl group) |
| End-capping | 있음 (고분자 엔드캡핑, Polymeric end-capping) |
| Target molecular weight | 30 kDa 이하 |
| pH range | pH 1.5 - 9 |
| Maximum pressure | 250 bar / 3,500 psi / 25 MPa |
| USP Code | L1 |
왜 기존 ODS 컬럼은 큰 분자에 약한가
최근 의약품은 구조가 복잡해지고 있고, 일부는 분자량이 1,000 Da를 넘는 경우도 있습니다. 이처럼 큰 분자는 기존 ODS 컬럼 (Pore size 10~15nm)에서 잘 분리되지 않습니다. 좁은 기공을 통과하기 어렵기 때문에 피크 모양이 흐려지고 분리 성능도 떨어지며, 작은 기공에서는 엔드캡핑도 충분히 이루어지지 않아 잔존 실라놀의 영향이 커지는 것도 원인입니다.
Wide Pore + 고분자 엔드캡핑의 결합
Cadenza CW-C18은 이런 문제를 해결한 컬럼입니다. 와이드 포어 (Wide Pore, 30 nm) 실리카를 사용해 큰 분자도 안정적으로 분리할 수 있습니다. 여기에 Cadenza CD-C18과 동일한 ODS 리간드 기술과, IMTAKT만의 고분자 엔드캡핑 (Polymeric End-Capping) 기술을 더해 안정성과 재현성을 확보했습니다.
짧아진 유지시간, 유지되는 소수성 인식력
CW-C18의 비표면적은 CD-C18의 약 1/3 수준으로, 동일 조건에서 유지 (Retention)는 짧아지지만, 소수성 인식 능력 (소수성 상호작용)은 동일하게 유지됩니다.
그 결과, 어떤 분석에 유리한가
- ●대분자·고소수성 화합물 (예: 인지질) 분석에 유리
- ●효율적인 엔드캡핑 처리로 염기성 화합물·항생제 분석 성능 개선
- ●100% 수계 이동상 사용 가능, 고극성 물질 분석에도 적합
즉, Cadenza CW-C18은 기존 ODS 컬럼의 한계를 넘어, 폭넓은 시료 분석을 지원하는 와이드 포어 ODS 컬럼입니다.
관련 애플리케이션 검색 (실시간 연동)
Cadenza CW-C18으로 진행된 모든 애플리케이션 데이터와 유저 리포트를 실시간으로 검색할 수 있습니다. (영문 사이트와 동일한 데이터베이스를 사용하여 새로운 자료가 등록되는 즉시 반영됩니다.)
대표 적용 사례
- ●Chloroquine 유사체 (항말라리아제)
- ●스핑고지질 (Sphingolipids, Ceramide) 분석 (MS)
- ●인지질 (Phospholipids) 분석 (MS)
- ●스핑고지질 (Sphingosine-1-phosphate) 분석 (MS)
- ●수용성 비타민 (Water-Soluble Vitamins) 분석
- ●폴리에틸렌글리콜 (PEG) #12000 #20000 분석
- ●염기성 화합물 (항우울제) 분석
- ●Omega-3 지방산 (DHA, EPA, AA) 용출 비교
그 외 더 많은 적용 사례는 애플리케이션 검색에서 확인하실 수 있습니다.
기술 자료 (Technical Information)
애플리케이션 (Applications)
최신 애플리케이션
- ● 클로로퀸 유사체 (항말라리아제)
- ● 카르복시페닐보론산 이성질체
- ● LC-MS: 니신, 나타마이신 (식품 보존료)
- ● 리그닌
- ● 피타바스타틴 (지질강하제) <중성 pH>
- ● 히드록시프로필 메틸셀룰로스 (HPMC)
- ● 카르바조크롬 (지혈제) 및 트록세루틴 (혈관 보호제)
- ● 폴리옥시에틸렌 라우릴 에테르 (비이온성 계면활성제)
- ● 플루바스타틴 (지질강하제)
- ● LC-MS: 오메가-3 및 오메가-6 지방산
- ● LC-MS: 오메가-3 지방산 에틸 에스테르
- ● LC-MS: 탄산탈수효소 억제제 (녹내장 치료제)
- ● LC-MS: 발리노마이신 (뎁시펩타이드, 항균제)
- ● LC-MS: 세레울라이드 (뎁시펩타이드, 세포독소)
- ● 글리코겐
- ● 이눌린
- ● 폴리스티렌설폰산
- ● 프로파게르마늄
- ● 계면활성제
- ● 폴리에틸렌 글리콜 (PEG)
- ● 폴리에틸렌 글리콜 (PEG)
- ● PEG화 단백질 (3)
- ● 말톨, 에틸 말톨
- ● 수용성 비타민
- ● 스핑고지질 (스핑가닌-1-인산)의 LC-MS/MS 응용
- ● 스핑고지질 (스핑고신-1-인산)의 LC-MS/MS 응용
- ● 스핑고지질 (스핑고신)의 LC-MS/MS 응용
- ● 스핑고지질 (세라마이드)의 LC-MS/MS 응용
- ● 인지질의 LC-MS 응용
- ● 표준 PA-당쇄
- ● 항생제 (에리스로마이신)
- ● 폴리펩타이드 항생제
- ● 염기성 화합물: 항우울제
- ● 할로아세트산류
- ● 트라이아릴메탄 잉크 염료의 초고속 분석
- ● 이중가닥 DNA 단편
유저 리포트 (User Reports)
-
●LC-MS Analysis of Sphingolipids
[Ohio State University, USA]
-
●Development of Sphingolipid Measurement System by LC-IT-TOF MS
[Gifu Pharmaceutical University]
-
●LC-TOFMS Analysis of Phospholipids
[University of Tokushima]
유저 논문·학술 자료 (User Articles)
Bioengineering of a Lactococcus lactis subsp. lactis strain enhances nisin production and bioactivity
Roxana Portieles, Hongli Xu, Feng Chen, Jingyao Gao, Lihua Du, Xiangyou Gao, Carlos Borroto Nordelo, Qiulin Yue, Lin Zhao, Nayanci Portal Gonzalez, Ramon Santos Bermudez, Orlando Borras-Hidalgo
PLOS ONE, April 10, 2023
https://doi.org/10.1371/journal.pone.0281175
LASP1, CERS6, and Actin Form a Ternary Complex That Promotes Cancer Cell Migration
Atsuko Niimi, Siripan Limsirichaikul, Keiko Kano, Yasuyoshi Mizutani, Toshiyuki Takeuchi, Patinya Sawangsri, Dat Quoc Tran, Yoshiyuki Kawamoto and Motoshi Suzuki
Cancers, 2023, 15, 2781
https://doi.org/10.3390/cancers15102781
Dietary and socioeconomic risk factors for fumonisin exposure among women of reproductive age in 18 municipalities in Guatemala from 2013 to 2014
Ariel V. Garsow, Olga R. Torres, Jorge A. Matute, Ronald T. Riley, Julie R. Harris, Archana P. Lamichhane, Orion McCotter, Barbara B. Kowalcyk
PLOS Global Public Health, August 9, 2022
https://doi.org/10.1371/journal.pgph.0000337
A REVIEW ON BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION BY HYPHENATED TECHNIQUES
Vyshali Veerareddy, Kumaraswamy Gandla
Journal of Pharmaceutical Negative Results, Volume13, Special Issue1, 2022, 35-40
DOI: 10.47750/pnr.2022.13.S01.05
CEBPγ facilitates lamellipodia formation and cancer cell migration through CERS6 upregulation
Hanxiao Shi, Atsuko Niimi, Toshiyuki Takeuchi, Kazuya Shiogama, Yasuyoshi Mizutani, Taisuke Kajino, Kenichi Inada, Tetsunari Hase, Takahiro Hatta, Hirofumi Shibata, Takayuki Fukui, Toyofumi Fengshi Chen-Yoshikawa, Kazuki Nagano, Takashi Murate, Yoshiyuki Kawamoto, Shuta Tomida, Takashi Takahashi, Motoshi Suzuki
Cancer Science. 2021;112:2770-2780
https://doi.org/10.1111/cas.14928
CERS6 required for cell migration and metastasis in lung cancer
Motoshi Suzuki, Ke Cao, Seiichi Kato, Naoki Mizutani, Kouji Tanaka, Chinatsu Arima, Mei Chee Tai, Norie Nakatani, Kiyoshi Yanagisawa, Toshiyuki Takeuchi, Hanxiao Shi, Yasuyoshi Mizutani, Atsuko Niimi, Tetsuo Taniguchi, Takayuki Fukui, Kohei Yokoi, Keiko Wakahara, Yoshinori Hasegawa, Yukiko Mizutani, Soichiro Iwaki, Satoshi Fujii, Akira Satou, Keiko Tamiya-Koizumi, Takashi Murate, Mamoru Kyogashima, Shuta Tomida, Takashi Takahashi
Journal of Cellular and Molecular Medicine, Volume24, Issue20, October 2020, Pages 11949-11959
https://doi.org/10.1111/jcmm.15817
Pyrrocidine, a molecular off switch for fumonisin biosynthesis
Minglu Gao, Anthony E. Glenn, Xi Gu, Trevor R. Mitchell, Timothy Satterlee, Mary V. Duke, Brian E. Scheffler, Scott E. Gold
PLOS Pathogens, July 6, 2020
https://doi.org/10.1371/journal.ppat.1008595
Vaticanol C, a phytoalexin, induces apoptosis of leukemia and cancer cells by modulating expression of multiple sphingolipid metabolic enzymes
Chisato Inoue, Sayaka Sobue, Naoki Mizutani, Yoshiyuki Kawamoto, Yuji Nishizawa, Masatoshi Ichihara, Toshiyuki Takeuchi, Fumihiko Hayakawa, Motoshi Suzuki, Tetsuro Ito, Yoshinori Nozawa, and Takashi Murate
Nagoya Journal of Medical Science, 82, 261-280, 2020
Occurrence of emerging mycotoxins in cereals and cereal-based products from the Korean market using LC-MS/MS
Dan-Bi Kim, Nho-Eul Song, Tae Gyu Nam, Sanghee Lee, Dongwon Seo & Miyoung Yoo
Food Additives & Contaminants: Part A, 36, 2, 289-295, 2019
Simultaneous quantification of tegoprazan and its major metabolite M1 in dog plasma using liquid chromatography-tandem mass spectrometry
Joohwan Kim, Sungjun Kim, Hyun-Jung Noh, Shin-Young Ryu, Soo-Jin Kim
Journal of Pharmaceutical and Biomedical Analysis, 171, 15, 99-103, 2019
In situ ergot alkaloid detection in three Balansia epichloe-infected grass species
C.W. Bacon, D.M. Hinton, T.R. Mitchell, E.R. Palencia
Journal of Applied Microbiology, 125, 4, 976-985, 2018
Assessment of Mycotoxins in Vitis vinifera Wines of the Southeastern United States
Stephanie L. Bolton, Trevor Mitchell, Phillip M. Brannen, Anthony E. Glenn
American Journal of Enology and Viticulture, 68, 336-343, 2017
Kinetic and thermodynamic characteristics of crystallization of vancomycin
Geon-Soo Ha. Jin-Hyun Kim
Korean Journal of Chemical Engineering, 34, 9, 2451-2458, 2017
Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
Hajime Miyaguchi
Journal of Visualized Experiments, 115, e54402, 2016
A Novel Population of Fusarium fujikuroi Isolated from Southeastern U.S. Winegrapes Reveals the Need to Re-Evaluate the Species’ Fumonisin Production
Stephanie L. Bolton, Phillip M. Brannen, Anthony E. Glenn
Toxins, 8(9), 254, 2016
Purification-Free Method for Preparing Technetium-99m-Labeled Multivalent Probes for Enhanced in Vivo Imaging of Saturable Systems
Yuki Mizuno, Tomoya Uehara, Hirofumi Hanaoka, Yota Endo, Chun-Wei Jen, and Yasushi Arano
Journal of Medicinal Chemistry, 59, 3331-3339, 2016
Elevated nuclear sphingoid base-1-phosphates and decreased histone deacetylase activity after fumonisin B1 treatment in mouse embryonic fibroblasts
Nicole M.Gardner, Ronald T.Riley, Jency L. Showker, Kenneth A.Voss, Andrew J.Sachs, Joyce R. Maddox, Janee B.Gelineau-van Waes
Toxicology and Applied Pharmacology, 298, 56-65, 2016
Targeting ceramide synthase 6-dependent metastasis-prone phenotype in lung cancer cells
Motoshi Suzuki, Ke Cao, Seiichi Kato, Yuji Komizu, Naoki Mizutani, Kouji Tanaka, Chinatsu Arima, Mei Chee Tai, Kiyoshi Yanagisawa, Norie Togawa, Takahiro Shiraishi, Noriyasu Usami, Tetsuo Taniguchi, Takayuki Fukui, Kohei Yokoi, Keiko Wakahara, Yoshinori Hasegawa, Yukiko Mizutani, Yasuyuki Igarashi, Jin-ichi Inokuchi, Soichiro Iwaki, Satoshi Fujii, Akira Satou, Yoko Matsumoto, Ryuichi Ueoka, Keiko Tamiya-Koizumi, Takashi Murate, Mitsuhiro Nakamura, Mamoru Kyogashima, and Takashi Takahashi
The Journal of Clinical Investigation, 126(1), 254-265, 2016
Improved Pretreatment Method for Determination of Nisins A and Z and Monitoring in Cheese Using Liquid Chromatrography-Tandem Mass Spectrometry
Kyung Yuk Ko, So Ra Park, Ho-Soo Lim, Se-Jong Park, Meehye Kim
Food Analytical Methods, 9, 1, 122-130, January 2016
A blood spot method for detecting fumonisin-induced changes in putative sphingolipid biomarkers in LM/Bc mice and humans
Ronald T Riley, Jency L Showker, Christine M Lee, Cody E Zipperer, Trevor R Mitchell, Kenneth A Voss, Nicholas C Zitomer, Olga Torres, Jorge Matute, Simon G Gregory, Allison E Ashley-Koch, Joyce R Maddox, Nicole Gardner, Janee B Gelineau-Van Waes
Food Addit Contam Part A Chem Anal Control Expo Risk Assess, 32, 6, 934-949, 2015
Effect of an ionic liquid on vancomycin crystallization
Geon Soo Ha, Jin-Hyun Kim
Korean Journal of Chemical Engineering, 32, 4, 576-582, April 2015
Investigation on the role of ion-exchange resin in the crystallization process for the purification of vancomycin
Sung-Jae Kim, Jin-Hyun Kim
Korean Journal of Chemical Engineering, 32, 3, 465-470, March 2015
Maize Seedling Blight Induced by Fusarium verticillioides: Accumulation of Fumonisin B1 in Leaves without Colonization of the Leaves
Thomas T. Baldwin, Nicholas C. Zitomer, Trevor R. Mitchell, Anne-Marie Zimeri, Charles W. Bacon, Ronald T. Riley, and Anthony E. Glenn
J. Agric. Food Chem., 62 (9), 2118-2125, 2014
Discovery of the surface polarity gradient on iridescent Morpho butterfly scales reveals a mechanism of their selective vapor response
Radislav A. Potyrailoa, Timothy A. Starkey, Peter Vukusic, Helen Ghiradella, Milana Vasudev, Timothy Bunning, Rajesh R. Naik, Zhexiong Tang, Michael Larsen, Tao Deng, Sheng Zhong, Manuel Palacios, James C. Grande, Gilad Zorn, Gregory Goddard, and Sergey Zalubovsky
PNAS, 110, 39, 15567-15572, 2013
ODS 系カラムによるビタミン C の高速液体クロマトグラフィー分析
永田 雅靖
日本食品科学工学会誌, 60, 2, 96-99, 2013
Effect of ion-exchange resin on increased surface area crystallization process for purification of vancomycin
Eun-A. Kwak, Sung-Jae Kim, Jin-Hyun Kim
Korean Journal of Chemical Engineering, 29, 11, 1487-1492, November 2012