Cadenza
Cadenza HS-C18 - 혈청 직접 주입, 친수성 폴리머 배제형 ODS 컬럼
Features
● 세계 최초의 친수성/소수성 하이브리드 ODS 컬럼 ● 샘플 전처리 없이 혈청 및 혈장 직접 주입 가능 ● 복잡한 시료 전처리 없이 희석 후 주입 가능 ● 친수성 고분자 배제 / 소수성 저분자 흡착 메커니즘 ● 혈액, 소변, 혈청 또는 세포 배양 추출물과 같은 생물학적 분석에 최적 ● LC-MS 호환성 우수
SPECIFICATIONS
Main Specifications
| Particle size | 3 μm |
|---|---|
| Base materials | 완전다공성 고순도 실리카겔 (Fully porous, high-purity silica gel) |
| Ligand | 옥타데실기, 친수성기 (Octadecyl group, hydrophilic group) |
| Target molecular weight | 약 30 kDa 이상은 배제, 이하는 흡착 |
| pH range | pH 2 - 8 |
| Maximum pressure | 250 bar / 3,500 psi / 25 MPa |
| USP Code | L1 |
기존 혈청 직접 분석 컬럼의 한계
기존의 혈청 직접 분석용 컬럼은 다음과 같은 한계를 지녔습니다.
- ●컬럼 스위칭을 위한 복잡한 LC 시스템 구성
- ●유기용매 농도 제약
- ●LC-MS 비호환성
세계 최초의 친수성/소수성 하이브리드 ODS
Cadenza HS-C18는 세계 최초의 친수성/소수성 하이브리드 ODS 컬럼으로, 혈청 단백질(예: 알부민)을 배제하면서 저분자 의약품을 소수성 상호작용으로 유지하고, 유기용매 그래디언트로 분리합니다.
혈청 전처리 없이, TDM까지
이를 통해 혈청 전처리를 단순화하며, 빠른 분석이 필요한 치료약물농도 모니터링(TDM)에 유용합니다. 또한 최근에는 단일클론항체 제제의 계면활성제·안정제 분석, 주사제와 같은 제형 내 의약품 및 친수성 폴리머 분리에도 널리 활용되고 있습니다.
다양한 분야로의 확장
Cadenza HS-C18은 단백질 및 친수성 폴리머 용액 속 저분자 물질의 LC-MS 분석을 가능하게 하여, 혈액 분석부터 제제 분석까지 다양한 분야에서 활용될 것으로 기대됩니다.
관련 애플리케이션 검색 (실시간 연동)
Cadenza HS-C18으로 진행된 모든 애플리케이션 데이터와 유저 리포트를 실시간으로 검색할 수 있습니다. (영문 사이트와 동일한 데이터베이스를 사용하여 새로운 자료가 등록되는 즉시 반영됩니다.)
대표 적용 사례
- ●항체 수용액 내 계면활성제 (Surfactants in Antibody Solutions)
- ●혈청 내 Minoxidil 분석
- ●소 혈청 내 항간질제 (Antiepileptic Drugs) 분석
- ●소 혈청 내 과불화알킬산 (Perfluoroalkyl Acids) 분석
- ●젤라틴(단백질) 용액 내 Calcitonin(펩타이드) 분석
그 외 더 많은 적용 사례는 애플리케이션 검색에서 확인하실 수 있습니다.
기술 자료 (Technical Information)
애플리케이션 (Applications)
유저 리포트 (User Reports)
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●Analysis of Surfactants in Monoclonal Antibody (mAb) Formulations
Amgen, USA
-
●LC-MS/MS Rapid Quantitative Method for Antiepileptic Drugs (AEDs) in Plasma
Inha University, KOREA
-
●Non-targeted Metabolomics by LC-QTOF MS
Korea Institute of Science and Technology, KOREA
-
●Development of a Novel Micafungin Plasma Concentration Measurement Method by Direct Serum Injection
Gifu Pharmaceutical University, JAPAN
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●Practicality of Plasma Direct Injection Method
Toa Pharmaceutical, JAPAN
유저 논문·학술 자료 (User Articles)
Quantitative analysis of residual aurintricarboxylic acid in biotherapeutic process streams using liquid chromatography triple quadrupole mass spectrometry
Ciaran Buckley, Ciara MacHale, Jonathan Bones
Journal of Pharmaceutical and Biomedical Analysis, Volume 238, 20 January 2024, 115798
Direct Quantitation of Poloxamer 188 in Adeno-Associated Virus Products Using Reversed-Phase Chromatography With Charged Aerosol Detection
Tai Nguyen, Zoran Sosic, Chong-Feng Xu
SEPARATION SCIENCE PLUS: Volume 7, Issue 9, September 2024, e202400045
https://doi.org/10.1002/sscp.202400045
Combined Use of Calcium-channel Blockers With Ombitasvir/Paritaprevir/Ritonavir Exacerbates Peripheral Edema in Elderly Japanese Patients
YUNA SADAKA, MIDORI SODA, AKIYO HORI, YURI MIYAHARA, YASUHISA OIDA, YOICHI NISHIGAKI, EIICHI TOMITA, TAKASHI MIZUI and KIYOYUKI KITAICHI
Anticancer Research, 42, 4, 2087-2093, 2022
Detection of 11-nor-9-carboxy-tetrahydrocannabinol in the hair of drug abusers by LC-MS/MS analysis
Han Soo Cho, Byungseok Cho, Juhyun Sim, Seung Kyung Baeck, Sangwhan In, Eunmi Kim
Forensic Science International, 295, 219-225, 2019
Change in the Binding of [11C]BU99008 to Imidazoline I2 Receptor Using Brain PET in Zucker Rats
Kazunori Kawamura, Tomoteru Yamasaki, Yiding Zhang, Hidekatsu Wakizaka, Akiko Hatori, Lin Xie, Masayuki Fujinaga & Ming-Rong Zhang
Molecular Imaging and Biology, 21, 105-112, 2019
*Radiosynthesis and in vivo evaluation of 11C-labeled BMS-193885 and its desmethyl analog as PET tracers for neuropeptide Y1 receptors
Kazunori Kawamura, Wakana Mori, Masayuki Fujinaga, Tomoteru Yamasaki, Yiding Zhang, Hidekatsu Wakizaka, Akiko Hatori, Lin Xie, Katsushi Kumata, Takayuki Ohkubo, Yusuke Kurihara, Masanao Ogawa, Nobuki Nengaki and Ming-Rong Zhang
EJNMMI Radiopharmacy and Chemistry volume 4, Article number: 4 (2019)
Development of a liquid chromatography/tandem mass spectrometry method for monitoring of long-term exposure to parabens
Sung-Hee Cho, Han-Na Song
Rapid Communications in Mass Spectrometry, 33, 1, 67-73, 2019
Developing new PET tracers to image the growth hormone secretagogue receptor 1a (GHS-R1a)
Kazunori Kawamura, Masayuki Fujinaga, Yoko Shimoda, Tomoteru Yamasaki, Yiding Zhang, Akiko Hatori, Lin Xie, Hidekatsu Wakizaka, Katsushi Kumata, Takayuki Ohkubo, Yusuke Kurihara, Masanao Ogawa, Nobuki Nengaki, Ming-Rong Zhang
Nuclear Medicine and Biology, 52, 49-56, 2017
Non-targeted metabolic profiling analysis for diagnosis of internet/smartphone addiction disorder
Hyuck Ho Son, Wan Soo Yun, Sung-Hee Cho
Analytical Science & Technology, 280, 2017
Direct injection LC-MS/MS method for the determination of teicoplanin in human plasma
Kwang-Youl Kima, Sang-Heon Choa, Yong-Hyun Songa, Moon-Suk Nam, Cheol-Woo Kim
J Chromatogr B, 1008, 125-131, 2016
*One-Pot Esterification and Amidation of Phenolic Acids
Kozo Nakamura, Takero Nakajima, Toshifumi Aoyama, Sho Okitsu and Masahiro Koyama
TETRAHEDRON, 70, 43, 8097-8107, 2014
Influence of Dosing Time on the Efficacy and Safety of Finasteride in Rats
Masafumi Kumazaki, Hitoshi Ando, Kentarou Ushijima, Tomohiro Maekawa, Yuya Motosugi, Makoto Takada, Masato Tateishi, and Akio Fujimura
THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 338, 2, 718-723, 2011
Direct determination of residual Pluronic F-68 in in-process samples from monoclonal antibody preparations by high performance liquid chromatography
Chung HH, Zhou C, Khor HK, Qiu J.
J Chromatogr A., 1218(15), 2106-2113, 2011
Direct-injection HPLC method of measuring micafungin in human plasma using a novel hydrophobic/hydrophilic hybrid ODS column.
Uranishi H, Nakamura M, Nakamura H, Ikeda Y, Otsuka M, Kato Z, Tsuchiya T.
J Chromatogr B, 879, 1029-1032, 2011
注射用エンドキサンの揮発性に関する調査
藪中裕美，坂口史明, 小林由佳, 中西弘和
医療薬学(Jpn. J. Pharm. Health Care Sci.), 36(5), 343-346, 2010
Influence of water and food consumption on inadvertent antibiotics intake among general population
Kyunghee Ji, Younglim Kho, Changu Park, Dohyeon Paek, Pandong Ryu, Domyung Paek, Minyoung Kim, Pangyi Kim, Kyungho Choi
Environmental Research, 110, 7, 641-649, 2010
Influence of a five-day vegetarian diet on urinary levels of antibiotics and phthalate metabolites: A pilot study with “Temple Stay” participants
Kyunghee Ji, Young Lim Kho, Yoonsuk Park, Kyungho Choi
Environmental Research, 110, 4, 375-382, 2010
Discovery of safety biomarkers for atorvastatin in rat urine using mass spectrometry based metabolomics combined with global and targeted approach
Bhowmik Salil Kumar, Young-Joo Lee, Hong Jae Yi, Bong Chul Chung, Byung Hwa Jung
Analytica Chimica Acta, 661, 1, 47-59, 2010
Sphingosine kinase isoforms regulate oxaliplatin sensitivity of human colon cancer cells through ceramide accumulation and AKT activation.
Nemoto S, Nakamura M, Osawa Y, Kono S, Itoh Y, Okano Y, Murate T, Hara A, Ueda H, Nozawa Y, Banno Y.
J Biol Chem, 284, 10422-10432, 2009
Biochemical, Functional, and Pharmacological Characterization of AT-56, an Orally Active and Selective Inhibitor of Lipocalin-type Prostaglandin D Synthase
Daisuke Irikura, Kosuke Aritake, Nanae Nagata, Toshihiko Maruyama, Shigeru Shimamoto and Yoshihiro Urade
J Biol Chem, 284, 7623-7630, 2009
Transcriptional regulation of neutral sphingomyelinase 2 gene expression of a human breast cancer cell line, MCF-7, induced by the anti-cancer drug, daunorubicin
Hiromi Ito, Masashi Murakami, Ayako Furuhata, Siqiang Gao, Kayo Yoshida, Sayaka Sobue, Kazumi Hagiwara, Akira Takagi, Tetsuhito Kojima, Motoshi Suzuki, Yoshiko Banno, Kouji Tanaka, Keiko Tamiya-Koizumi, Mamoru Kyogashima, Yoshinori Nozawa, Takashi Murate
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 1789, 11-12, 681-690, 2009
Implications of sphingosine kinase 1 expression level for the cellular sphingolipid rheostat: relevance as a marker for daunorubicin sensitivity of leukemia cells.
Sobue S, Nemoto S, Murakami M, Ito H, Kimura A, Gao S, Furuhata A, Takagi A, Kojima T, Nakamura M, Ito Y, Suzuki M, Banno Y, Nozawa Y, Murate T.
Int J Hematol, 87, 266-275, 2008